Sodium Pyruvate Powder

Sodium Pyruvate Powder – High-Purity Biochemical Intermediate for Cell Culture & Pharmaceutical Applications

Xi'an Le-Nutra Ingredients Inc provides high quality Sodium Pyruvate Powder (Sodium 2-oxopropanoate, CAS 113-24-6) with purity ≥99% by HPLC. This white to off-white crystalline powder is produced at our 60,000㎡ GMP-certified production facility. It is an important energy metabolism intermediate used in cell culture media, pharmaceutical synthesis and biochemical research. We produce 3,000 tonnes per year and have sophisticated quality control systems that assure constant performance from batch to batch. We have over 14 years of experience producing functional ingredients. Full documentation (COA, TDS, MSDS, HPLC chromatogram) is supplied with each shipment to meet global regulatory standards for laboratory, diagnostic and industrial applications.

What Is Sodium Pyruvate Powder?

It is the sodium salt derivative of pyruvic acid, a three-carbon α-keto acid that lies at the center of both glycolysis and the citric acid cycle. Molecular formula C₃H₃NaO₃. Molecular weight 112.06. Direct energy substrate for cellular respiration. Versatile building block in organic production.

This biological molecule solves important issues in biomanufacturing and research contexts. Traditional cell culture mediums that only include glucose may lead to lactate accumulation and metabolic acidosis during high density fermentation. Supplemental sodium pyruvate gets around this barrier by entering metabolism downstream of the glycolytic pathway, thereby allowing appropriate pH levels and increasing ATP generation. Its antioxidant qualities also protect cultured cells against oxidative stress generated by hydrogen peroxide, increasing viability up to 30% in sensitive cell types.

In diagnostic use, the molecule is the substrate of choice for lactate dehydrogenase (LDH) tests, allowing precise assessment of alanine aminotransferase (ALT) activity in liver function testing. Pharmaceutical firms appreciate it as a clean starting material for the synthesis of antiviral medicines, cardiovascular medications, and amino acid derivatives.

Technical Specifications & Quality Parameters

We have a robust testing methodology that comprises HPLC assay verification, Karl Fischer titration for water content, ICP-MS heavy metal screening and pH measurement in a controlled environment. All batches are microbiologically tested per USP standards to assure safety for pharmaceutical and cell culture use.

Core Functional Advantages

Energy Metabolism Assistance

This powder is a direct substrate for the citric acid cycle, therefore bypassing glycolytic constraints and allowing fast ATP synthesis. It has been shown to enhance cellular energy stores by 22% over glucose-only media and to be especially useful for hybridoma cultures and primary cell expansion.

Improved Antioxidant Defence

The chemical scavenges reactive oxygen species (ROS) by its keto group and protects cells from H 2 O 2 -induced apoptosis. It is therefore an essential component of serum free and chemically defined media formulations. Studies suggest it decreases signs of oxidative damage in stressed fibroblast cells by 40%.

Versatility of Pharmaceutical Synthesis

Its clear reactivity profile allows for the selective synthesis of chiral amino acids, antiviral nucleoside analogues, and cardiovascular medicines. The sodium salt form avoids hygroscopicity problems of free pyruvic acid, enhancing safety in handling and consistency of reaction.

Diagnostic Precision

In linked enzyme assays with NADH, it produces linear response curves for quantification of ALT with detection limits < 5 U/L, consistent with clinical laboratory standards for liver panel testing.

Primary Application Scenarios

Biopharmaceutical Cell Culture

Add to basal media at concentrations of 0.5-1.0 mM to increase productivity of CHO, HEK293 and Vero cells. Especially effective in high density fed-batch fermentation where lactate buildup restricts development. When paired with optimised feeding techniques, users have reported 15-25% increases in monoclonal antibody production.

Diagnostic Reagent Production

Formulated as the principal substrate in ALT/LDH assay kits. The high purity and the low amounts of endotoxin (<0.1 EU/mg) ensure reproducible development of colorimetric and fluorometric signals. Stable in freeze-dried state for room temperature storage in kits .

Synthesis of Pharmaceutical Intermediates

Serve as the C3 building block for the synthesis of L-DOPA, alanine derivatives and β-lactam precursors. Sodium salt form decreases corrosion hazards in stainless steel reactors and maintains >95% conversion efficiency under moderate conditions.

Cosmetic Formulations

Incorporate into anti-aging serums and recovery creams at concentrations of 0.1-0.5%. It is tiny enough to penetrate the skin and promote the energy metabolism in fibroblasts and the collagen formation pathways.

Quality Assurance & Compliance

Our production facility operates under ISO 9001, ISO 22000, and GMP certifications with annual third-party audits. Each manufacturing batch undergoes 18-point quality verification including:

• Identity confirmation via NMR spectroscopy
• Quantitative purity by validated HPLC methods
• Elemental impurity screening per ICH Q3D guidelines
• Residual solvent analysis (meets Ph.Eur. limits)
• Sterility and endotoxin testing for cell culture grades

Certificates of Analysis contain batch-specific data, stability studies, and regulatory compliance assertions. We maintain full traceability from raw material sourcing through final packaging, and electronic batch records are archived for 10+ years.

Packaging & Storage Conditions

Standard Packaging:

• 25 kg fiber drums with double-layer food-grade polyethylene liners
• 1 kg / 5 kg / 10 kg aluminum foil bags for R&D quantities

Customisation Options:

Small-volume glass vials for analytical standards, bulk bags for industrial scale formulation and aseptic packaging for cell culture applications.

Storage Needs:

Keep containers securely closed. Store at 2-8°C. Store in a dry, light and oxidising agent free environment. Sodium Pyruvate Powder is stable for 24 months under suitable settings at or below room temperature. Open containers should be purged with nitrogen and utilised with in 6 months.

Why Partner With Xi'an Le-Nutra?

Factory Direct Price

Eliminate distributor markups with our vertically integrated manufacturing approach. We offer tiered pricing for bulk orders but keep our quality high.

Configurable

Our R&D team works together to modify specifications such as ultrahigh purity grades (99.5% +), particle size distribution control and bespoke analytical procedures. OEM services include private labelling and co-packaging.

Global Distribution Network

We do maritime freight, air express and temperature controlled shipping to 60+ countries. Stock products have an average lead time of 10-15 business days and have real time monitoring support.

Technical Advice

Our biochemistry experts can help you with formulation, advice on stability testing and support with regulatory documentation throughout your product development lifecycle.

FAQ

Q: What makes this substance so special as compared to ordinary pyruvate salts?

A: Our ≥99% purity criteria excludes trace organic acids and inorganic salts that interfere with enzymatic tests and reproducibility in cell culture. Third party verification shows <10 ppm transition metals, which are crucial to prevent catalytic degradation in formulations.

Q: What is the mechanism of protection against oxidative stress?

A: The α-keto structure interacts directly with hydrogen peroxide to generate acetate and CO₂, neutralising ROS without creating free radicals. This non-enzymatic scavenging complements antioxidant enzyme systems such as catalase and superoxide dismutase.

Q: Can it replace glucose completely in culture media?

A: It is an effective energy source yet most cell types require glucose for metabolic pathways (nucleotide synthesis, pentose phosphate pathway) . For balanced energy generation and biosynthesis, the optimal performance is achieved by adding 10-25 mM glucose and 1-2 mM product.

Q: What paperwork supports the pharmacological use?

A: We supply drug master files (DMF) for regulatory filings. These files describe the manufacturing process, impurity profiles, and stability data in accordance with ICH requirements. Material Safety Data Sheets (MSDS) and Certificates of Suitability available on request.

Q: What is the delivery time for bulk orders ?

A: Max 5 tonnes stock in inventory for quick shipping. Custom manufacturing runs (>10 tonnes) require a 3-4 week lead time, however we can be flexible to meet your project timelines.

Request Your Sample Today

Contact us at enquiry@lenutra.com to request Sodium Pyruvate Powder samples, discuss specifications, or explore partnership opportunities.